Widely used in oligonucleotide synthesis, this compound enables the incorporation of protected guanine nucleotides into DNA strands, particularly in automated solid-phase synthesis. Its isobutyryl protection stabilizes the exocyclic amine during coupling, minimizing side reactions. The FDMT group at the 5'-end offers acid-labile protection, allowing selective deprotection under mild conditions, which is crucial for synthesizing long or complex sequences. The phosphoramidite functionality ensures high coupling efficiency, making it ideal for producing primers, probes, and modified nucleic acids used in PCR, sequencing, and antisense technologies. Its reliability and performance support high-yield synthesis in both research and pharmaceutical development settings.