Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the site-specific incorporation of 8-oxoguanosine—a modified nucleoside that mimics oxidative DNA damage. It is particularly valuable in preparing DNA probes for studying DNA repair mechanisms, mutagenesis, and oxidative stress-related biological pathways. The DMT and phosphoramidite protecting groups ensure efficient coupling and stepwise chain elongation on automated synthesizers. The 5′-O-DMT group allows for purification by trityl-on methods, while the isobutyryl protection on the exocyclic amine ensures stability during synthesis. Widely applied in the production of modified antisense strands, diagnostic probes, and epigenetic research tools.