Used in solid-phase oligonucleotide synthesis to introduce a guanosine residue in RNA constructs, with the 2'-OH protected by the TC group to prevent side reactions and enable synthesis of longer RNA chains, and the base protected by isobutyryl to avoid unwanted reactions. The phosphoramidite moiety at the 3' position facilitates efficient nucleoside coupling, while the DMT group at the 5' position allows for controlled, stepwise elongation and purification using trityl-on techniques. Commonly applied in the production of siRNA, mRNA, antisense oligonucleotides, diagnostic probes, and other RNA-based tools for molecular biology research and therapeutic development.