Widely used in oligonucleotide synthesis, this compound enables the incorporation of modified guanosine into DNA strands. The N2-methylguanosine modification enhances stability and influences base-pairing properties, making it valuable in the development of antisense oligonucleotides, PCR primers, and probes where precise hybridization control is required. The DMT group allows for stepwise chain elongation in solid-phase synthesis by providing reversible protection, while the phosphoramidite group ensures efficient coupling. Its application is critical in research involving gene regulation, nucleic acid therapeutics, and functional genomics.