Used primarily as an analytical reagent in biochemical and organic chemistry for the detection and quantification of esterase enzyme activity. The compound acts as a substrate that, upon enzymatic hydrolysis, releases 2,4-dinitrophenol—a yellow-colored product that can be easily measured spectrophotometrically. This property makes it valuable in enzyme kinetics studies and in screening for esterase-producing microorganisms. It is also employed in the development and testing of biosensors and in environmental monitoring to assess enzyme activity in soil and water samples. Due to its chromogenic response, it is favored in colorimetric assays where visual or instrumental detection of enzyme action is required.