Used as a cationic lipid in non-viral gene delivery systems, enabling efficient transfection of DNA and RNA into cells. Its positive charge interacts with negatively charged nucleic acids, forming lipoplexes that fuse with cell membranes to enhance cellular uptake. Commonly applied in research for gene therapy, vaccine development, and functional genomics. Also utilized in liposomal formulations to improve stability and delivery efficiency in both in vitro and in vivo settings. Shows particular value in mRNA-based therapeutic platforms due to its ability to support rapid and transient gene expression.