Dynole 34-2 is primarily used in cellular and molecular biology research as a potent inhibitor of dynamin, a protein involved in endocytosis. By blocking dynamin's GTPase activity, it effectively halts clathrin-mediated endocytosis, allowing researchers to study the role of endocytosis in various cellular processes. It is commonly applied in studies related to membrane trafficking, receptor internalization, and synaptic vesicle recycling. Due to its rapid and reversible action, Dynole 34-2 is valuable for time-sensitive experiments where precise control over endocytic pathways is required. It has also been used to investigate viral entry mechanisms, as many viruses rely on endocytosis to enter host cells.