Used as a fluorescent labeling reagent in bioconjugation and bioimaging applications. Its aggregation-induced emission (AIE) properties make it highly effective for tracking biomolecules in aqueous environments, where traditional fluorophores often quench. The active ester group readily reacts with primary amines on proteins, peptides, or amine-modified nucleic acids, forming stable amide bonds. This allows for efficient and specific labeling of biomolecules without the need for catalysts. Due to the triphenylvinyl group, it exhibits strong fluorescence upon binding to macromolecules or in hydrophobic environments, enabling sensitive detection in cellular imaging and diagnostic assays. Also employed in the development of AIE-based sensors and probes for monitoring biological processes in real time.