Used as an activated ester in bioconjugation and peptide synthesis, enabling efficient coupling with primary amines under mild conditions. Its alkyne group allows for subsequent click chemistry reactions, making it valuable in the development of bioconjugates, drug delivery systems, and functionalized biomolecules. Commonly employed in the preparation of protein-protein or protein-small molecule conjugates where selective and sequential modifications are required. Also utilized in materials science for surface functionalization and in the synthesis of dendrimers and other branched macromolecular structures.