c-Fms-IN-3 is primarily used in research settings to study and inhibit the activity of the colony-stimulating factor-1 receptor (CSF-1R), also known as c-Fms. This receptor plays a critical role in the regulation of macrophages and other immune cells. By targeting c-Fms, this compound helps researchers investigate its involvement in diseases such as cancer, inflammatory disorders, and autoimmune conditions. In cancer research, it is particularly valuable for exploring tumor-associated macrophages (TAMs), which contribute to tumor growth and immune evasion. Inhibiting c-Fms with this compound can provide insights into potential therapeutic strategies to modulate the tumor microenvironment and enhance anti-cancer immune responses. Additionally, it aids in understanding the mechanisms of macrophage-dependent inflammation, offering potential pathways for treating chronic inflammatory diseases.