PF-573228 is primarily used in biochemical research as a potent and selective inhibitor of focal adhesion kinase (FAK). It is employed to study the role of FAK in cellular processes such as cell adhesion, migration, and survival. Researchers utilize this compound to investigate its potential in inhibiting cancer cell proliferation and metastasis, as FAK is often overexpressed in various cancers. Additionally, PF-573228 is used in preclinical studies to explore its effects on angiogenesis and tumor growth, providing insights into potential therapeutic applications in oncology. Its ability to block FAK signaling pathways makes it a valuable tool for understanding cellular mechanisms and developing targeted cancer therapies.