PF-431396 is primarily used in research settings as a potent inhibitor of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2). Its application is significant in studying cellular processes such as cell adhesion, migration, and survival, which are crucial in understanding cancer biology. Researchers utilize PF-431396 to investigate the role of FAK and Pyk2 in tumor progression and metastasis, as these kinases are often overexpressed in various cancers. Additionally, it aids in exploring potential therapeutic strategies targeting FAK/Pyk2 signaling pathways to inhibit cancer cell growth and invasion. The compound is also valuable in studying other FAK/Pyk2-related physiological and pathological processes, including wound healing and inflammation.