PF-00562271 is primarily utilized in research settings as a potent inhibitor of focal adhesion kinase (FAK), a protein involved in cell adhesion, migration, and survival. Its application is significant in studying cancer biology, as FAK is often overexpressed in various tumor types, contributing to tumor growth, metastasis, and resistance to therapy. By inhibiting FAK, PF-00562271 helps researchers investigate its role in cancer progression and evaluate its potential as a therapeutic target. Additionally, it is used in preclinical studies to explore its effects on other FAK-dependent processes, such as angiogenesis and inflammation, providing insights into broader therapeutic applications beyond oncology.