TNK2-IN-1 is primarily utilized in biochemical research as a potent and selective inhibitor of TNK2 (tyrosine kinase non-receptor 2), also known as ACK1. Its application is focused on studying the role of TNK2 in various cellular processes, particularly in cancer research. By inhibiting TNK2, researchers can investigate its involvement in signaling pathways that regulate cell proliferation, survival, and migration. This compound is valuable in understanding the mechanisms of TNK2 in tumorigenesis and exploring its potential as a therapeutic target in cancers where TNK2 is overexpressed or dysregulated. Additionally, TNK2-IN-1 is used in preclinical studies to evaluate the efficacy of TNK2 inhibition in reducing tumor growth and metastasis, providing insights for developing targeted cancer therapies.