GNF5-amido-Me is primarily utilized in the field of biomedical research, particularly in the study of kinase signaling pathways. It serves as a selective inhibitor targeting specific kinases, which are crucial in cellular processes such as proliferation, differentiation, and apoptosis. This compound is often employed in laboratory settings to investigate the role of these kinases in disease mechanisms, especially in cancer research. By inhibiting kinase activity, GNF5-amido-Me helps researchers understand the potential therapeutic effects of kinase modulation, aiding in the development of targeted cancer therapies. Additionally, it is used in high-throughput screening assays to identify and validate new drug candidates that can selectively inhibit kinase activity, contributing to the advancement of precision medicine.