K252a is widely used in biochemical research as a potent inhibitor of protein kinases, particularly those involved in cell signaling pathways. It is extensively applied in studies focused on understanding the role of nerve growth factor (NGF) and its receptor, TrkA, in neuronal development and survival. Researchers utilize K252a to investigate mechanisms of neuroprotection, neurodegeneration, and differentiation in various cell models. Additionally, it serves as a valuable tool in cancer research to explore kinase-dependent pathways that regulate cell proliferation and apoptosis. Its ability to modulate kinase activity makes it a critical compound for studying cellular processes and developing potential therapeutic strategies.