AG 1296 is primarily used in research settings to study cellular signaling pathways. It acts as a selective inhibitor of platelet-derived growth factor receptor (PDGFR) tyrosine kinase, making it valuable for investigating the role of PDGFR in various biological processes. Researchers utilize AG 1296 to explore its effects on cell proliferation, migration, and angiogenesis, particularly in cancer studies. By blocking PDGFR signaling, it helps in understanding the mechanisms underlying tumor growth and metastasis. Additionally, it is employed in experiments to examine the therapeutic potential of PDGFR inhibition in diseases such as fibrosis and atherosclerosis. Its application extends to in vitro and in vivo models to assess its efficacy and specificity in modulating cellular responses.