Tyrosine kinase-IN-1 is primarily utilized in research settings to study the inhibition of tyrosine kinase enzymes, which play a critical role in cell signaling and cancer progression. It is often employed in biochemical assays to understand the mechanisms of kinase activity and to evaluate potential therapeutic strategies for diseases driven by abnormal kinase signaling, such as certain cancers. Researchers use this compound to investigate its effects on tumor growth and metastasis, providing insights into the development of targeted cancer therapies. Additionally, it serves as a tool compound in drug discovery to identify and optimize new kinase inhibitors with improved efficacy and selectivity.