PF-562271 HCl is primarily utilized in research settings as a potent and selective inhibitor of focal adhesion kinase (FAK). Its application is significant in studying cellular processes such as cell adhesion, migration, and survival, which are crucial in understanding cancer biology. Researchers use this compound to explore the role of FAK in tumor growth and metastasis, providing insights into potential therapeutic targets. Additionally, it aids in investigating signaling pathways involved in angiogenesis and wound healing, offering a tool to dissect the molecular mechanisms underlying these processes. Its use in preclinical studies helps evaluate the efficacy of FAK inhibition in various cancer models, contributing to the development of novel anticancer strategies.