ML204 hydrochloride is primarily used in research settings as a selective antagonist of the TRPC4/TRPC5 channels, which are involved in calcium signaling pathways. Its application is significant in studying the physiological and pathological roles of these channels in various tissues, including the nervous system, cardiovascular system, and smooth muscle. Researchers utilize ML204 hydrochloride to investigate its potential therapeutic effects in conditions such as anxiety, depression, and cardiovascular diseases. Additionally, it aids in understanding the mechanisms underlying channel-related disorders and in developing targeted treatments. Its specificity makes it a valuable tool for elucidating the complex functions of TRPC4/TRPC5 channels in cellular processes.