RN-1734 is primarily used in research settings as a selective TRPV4 channel antagonist. It is widely applied in studies focused on understanding the role of TRPV4 channels in various physiological and pathological processes. Researchers utilize RN-1734 to investigate its effects on cellular mechanisms, such as calcium signaling, osmoregulation, and mechanical sensation. It is particularly valuable in exploring conditions like pain, inflammation, and edema, where TRPV4 channels are implicated. Additionally, RN-1734 aids in the development of potential therapeutic strategies targeting TRPV4-related disorders. Its application extends to in vitro and in vivo models, providing insights into channel function and potential drug interactions.