ML204 is primarily utilized in research settings as a selective antagonist for the TRPC4/TRPC5 channels, which are part of the transient receptor potential cation channel family. Its application is significant in studying the physiological and pathological roles of these channels in various cellular processes. Researchers employ ML204 to investigate its effects on neuronal excitability, smooth muscle contraction, and its potential implications in neurological disorders, cardiovascular diseases, and kidney function. By inhibiting TRPC4/TRPC5 channels, ML204 helps elucidate their contribution to cellular calcium signaling and their involvement in disease mechanisms, paving the way for potential therapeutic developments.