ANA-12 is primarily used in research settings to study the role of the TrkB receptor in various neurological processes. It acts as a selective antagonist of TrkB, making it a valuable tool for investigating the mechanisms of brain-derived neurotrophic factor (BDNF) signaling. Researchers utilize ANA-12 to explore its effects on neuroplasticity, learning, memory, and mood regulation. It has also been employed in studies related to neurodegenerative diseases, depression, and anxiety disorders, helping to elucidate the potential therapeutic implications of modulating TrkB activity. Additionally, ANA-12 is used to examine the relationship between BDNF signaling and pain perception, offering insights into chronic pain management strategies. Its application extends to understanding the role of TrkB in cancer, as aberrant BDNF signaling has been implicated in tumor growth and progression.