DL-threo-β-Hydroxyaspartic acid is primarily used in biochemical research as a competitive inhibitor of glutamate transporters. It helps in studying the mechanisms of glutamate uptake in neural cells, which is crucial for understanding neurological disorders such as epilepsy, stroke, and neurodegenerative diseases. Additionally, it serves as a building block in the synthesis of peptides and other complex organic molecules, aiding in the development of pharmaceuticals and bioactive compounds. Its role in modulating glutamate activity also makes it valuable in exploring potential therapeutic strategies for conditions linked to glutamate dysregulation.