SEA-0400 is primarily utilized in research settings to study cellular processes involving sodium-calcium exchangers (NCX). It is a potent and selective inhibitor of NCX, making it valuable for investigating the role of these exchangers in various physiological and pathological conditions. Researchers use SEA-0400 to explore its effects on cardiac function, particularly in conditions like ischemia-reperfusion injury, where NCX activity plays a critical role. Additionally, it is employed in studies related to neuronal function and calcium homeostasis, helping to understand its implications in neurodegenerative diseases. Its application extends to pharmacological studies aimed at developing therapeutic strategies targeting NCX-related pathways.