EIPA hydrochloride is widely used in biological research as a potent inhibitor of sodium-hydrogen exchangers (NHEs). It is particularly valuable in studying cellular processes involving pH regulation, as it effectively blocks NHE activity, which plays a critical role in maintaining intracellular pH balance. This compound is often employed in experiments investigating cell proliferation, apoptosis, and migration, especially in cancer research, where altered pH regulation is a hallmark of tumor cells. Additionally, EIPA hydrochloride is utilized in studies of ischemia-reperfusion injury, as NHE inhibition can help mitigate cellular damage caused by pH imbalances during oxygen deprivation. Its application extends to cardiovascular research, where it aids in understanding the role of NHEs in heart function and disease.