NHE3-IN-1 is primarily used in research settings to study the role of the sodium-hydrogen exchanger 3 (NHE3) in various physiological and pathological processes. It acts as an inhibitor of NHE3, making it a valuable tool for investigating its function in electrolyte and fluid balance within the kidneys and gastrointestinal tract. Researchers utilize this compound to explore its potential therapeutic applications in conditions such as hypertension, heart failure, and diarrhea, where NHE3 activity is dysregulated. Additionally, it helps in understanding the mechanisms of drug interactions and the development of targeted therapies for diseases involving NHE3. Its application extends to preclinical studies aimed at evaluating the efficacy and safety of NHE3 inhibition in animal models.