Asp-AMS is primarily used in biochemical research as a potent and selective inhibitor of aminoacyl-tRNA synthetases, particularly aspartyl-tRNA synthetase. This enzyme plays a critical role in protein synthesis by attaching aspartic acid to its corresponding tRNA. By inhibiting this enzyme, Asp-AMS effectively halts protein synthesis, making it a valuable tool for studying cellular processes and mechanisms of translation. It is also utilized in the development of antimicrobial agents, as targeting aminoacyl-tRNA synthetases can disrupt bacterial protein synthesis, offering potential therapeutic applications against bacterial infections. Additionally, Asp-AMS is employed in structural biology to investigate enzyme-substrate interactions and to aid in the design of novel inhibitors for drug discovery.