eIF4A3-IN-1 is primarily utilized in biomedical research to study the role of eIF4A3 in RNA metabolism and its involvement in cancer progression. It serves as a potent inhibitor of eIF4A3, a key component of the exon junction complex, which is crucial for mRNA splicing, translation, and nonsense-mediated decay. Researchers use this compound to investigate the effects of eIF4A3 inhibition on tumor growth and survival, particularly in cancers where dysregulated RNA processing is a hallmark. Additionally, it aids in understanding the molecular mechanisms underlying eIF4A3's function in cellular processes and its potential as a therapeutic target for cancer treatment.