eIF4A3-IN-2 is primarily used in research settings to study the role of eIF4A3 in RNA metabolism and splicing. It serves as a selective inhibitor of eIF4A3, a helicase involved in the exon junction complex (EJC), which is crucial for post-transcriptional gene regulation. By inhibiting eIF4A3, researchers can investigate its impact on mRNA processing, translation, and nonsense-mediated decay (NMD). This compound is particularly valuable in understanding the molecular mechanisms underlying diseases where RNA splicing and regulation are disrupted, such as cancer and genetic disorders. Additionally, it aids in exploring potential therapeutic targets by modulating eIF4A3 activity in cellular and biochemical assays.