Widely used in molecular biology for efficient transfection of nucleic acids into mammalian cells, especially useful for delivering DNA, RNA, and siRNA into hard-to-transfect cell types. Its positive charge allows it to form complexes with negatively charged nucleic acids, facilitating cellular uptake through endocytosis. Commonly applied in gene expression studies, RNA interference experiments, and protein production workflows. Suitable for both transient and stable transfection in adherent and suspension cells. Often used in serum-containing media, reducing the need for media changes during transfection, which simplifies protocols and improves cell viability. Also employed in vaccine development and functional genomics due to its high efficiency and reproducibility.