AS1842856 is primarily utilized in research settings to study cellular signaling pathways, particularly those involving the FOXO1 transcription factor. It is widely employed as a potent and selective inhibitor of FOXO1 activity, enabling scientists to investigate the role of FOXO1 in various biological processes, such as metabolism, cell cycle regulation, and apoptosis. This compound is particularly valuable in understanding the mechanisms underlying diseases like diabetes, cancer, and neurodegenerative disorders, where FOXO1 plays a critical role. Additionally, AS1842856 is used in drug discovery efforts to identify potential therapeutic targets by modulating FOXO1-related pathways. Its application extends to in vitro and in vivo studies, providing insights into gene expression regulation and cellular responses to stress and growth factors.