Used in bioconjugation applications, particularly in click chemistry due to the presence of the TCO (trans-cyclooctene) group, which enables fast, catalyst-free reactions with tetrazine-modified biomolecules. The PEG3 spacer enhances solubility and reduces steric hindrance, improving reaction efficiency in biological systems. The Gly-Gly-Gly tripeptide segment can act as a cleavable linker or be recognized by certain enzymes, making it useful in prodrug designs or targeted delivery systems. Commonly employed in the development of antibody-drug conjugates (ADCs), imaging probes, and targeted therapeutics where controlled release and high specificity are required.