Used in molecular biology and biochemistry for tracking and visualizing the migration of DNA, RNA, and proteins during gel electrophoresis. The dye helps monitor the progress of electrophoresis runs, ensuring accurate separation and analysis of biomolecules. Its Ficoll-based formulation enhances sample loading by increasing density, allowing samples to sink properly into gel wells. Commonly applied in techniques like SDS-PAGE, agarose gel electrophoresis, and other separation methods to provide clear reference points for molecular weight estimation and migration consistency.