HI-TOPK-032 is primarily utilized in biochemical research as a potent and selective inhibitor of T-LAK cell-originated protein kinase (TOPK). Its application is significant in studying the role of TOPK in various cellular processes, including cell proliferation, apoptosis, and DNA damage response. Researchers employ HI-TOPK-032 to investigate its potential in cancer therapy, as TOPK is often overexpressed in certain types of cancer cells. By inhibiting TOPK, this compound helps in understanding the pathways involved in tumor growth and progression, providing insights for developing targeted cancer treatments. Additionally, it is used in experimental models to explore its effects on reducing tumorigenesis and enhancing the efficacy of existing chemotherapeutic agents.