TLR4-IN-C34 is primarily utilized in research focused on inflammation and immune response modulation. It acts as an inhibitor of Toll-like receptor 4 (TLR4), which plays a critical role in the activation of innate immunity. By blocking TLR4, this compound helps in studying pathways involved in inflammatory diseases such as sepsis, rheumatoid arthritis, and other autoimmune conditions. Researchers also explore its potential in reducing neuroinflammation, which is implicated in neurodegenerative disorders like Alzheimer's disease. Additionally, TLR4-IN-C34 is used in preclinical studies to understand its effects on cancer progression, as TLR4 signaling can influence tumor growth and metastasis. Its application extends to investigating therapeutic strategies for conditions where excessive TLR4 activation is detrimental.