EGTA is widely used in biochemistry and molecular biology as a chelating agent, particularly for binding calcium ions. It is employed in experiments where precise control of calcium concentration is crucial, such as in studying calcium-dependent enzymes, signaling pathways, and cellular processes. EGTA's high selectivity for calcium over magnesium makes it valuable in isolating calcium's role in biological systems. Additionally, it is used in buffer solutions to maintain low calcium levels, ensuring accurate experimental conditions. In medical research, EGTA aids in investigating calcium's involvement in muscle contraction, neurotransmitter release, and other physiological functions. Its ability to chelate calcium also finds applications in industrial processes, such as water treatment and metal ion removal.