EDTA disodium salt dihydrate is widely used in various industries due to its chelating properties. In the food industry, it acts as a preservative by binding metal ions that can catalyze oxidation, thereby extending the shelf life of products. It is also employed in the medical field as an anticoagulant in blood collection tubes, preventing blood clotting by sequestering calcium ions.

In water treatment, it helps to remove heavy metal ions from wastewater, making it safer for discharge into the environment. The chemical is also utilized in the cosmetics industry to stabilize formulations by preventing metal ion-induced degradation of ingredients. Additionally, it finds application in the textile industry as a dyeing auxiliary, ensuring even color distribution by controlling metal ions in the dye bath.

In agriculture, EDTA disodium salt dihydrate is used to deliver essential micronutrients to plants by forming soluble complexes with metal ions, enhancing nutrient uptake. It also plays a role in cleaning products, where it helps to soften water by binding calcium and magnesium ions, improving the effectiveness of detergents. Overall, its ability to chelate metal ions makes it a versatile compound across multiple sectors.