EDTA tripotassium salt is widely used in various industries due to its strong chelating properties. In medical and pharmaceutical applications, it is utilized as an anticoagulant in blood collection tubes, preventing blood clotting by binding calcium ions. It is also employed in chelation therapy to treat heavy metal poisoning, as it effectively binds and removes toxic metals like lead and mercury from the body.

In the food industry, it serves as a preservative and stabilizer, preventing spoilage by sequestering metal ions that catalyze oxidation and degradation processes. It is commonly added to canned foods, dressings, and beverages to maintain color, flavor, and texture.

In laboratory settings, EDTA tripotassium salt is used as a reagent in molecular biology and biochemistry. It is essential for DNA and RNA extraction, as it inhibits nucleases by chelating magnesium ions, ensuring the integrity of nucleic acids. Additionally, it is used in buffer solutions to maintain stable pH levels during experiments.

In industrial applications, it is employed in water treatment processes to control water hardness by binding calcium and magnesium ions, preventing scale formation in pipes and boilers. It is also used in cleaning products and detergents to enhance their effectiveness by sequestering metal ions that interfere with cleaning agents.

Furthermore, in the cosmetics industry, it acts as a stabilizer to prevent oxidation and maintain product integrity. In agriculture, it is incorporated into fertilizers and soil conditioners to improve nutrient uptake by chelating metals that might otherwise inhibit absorption.