Used extensively as a chelating agent in biochemistry and molecular biology to bind and sequester metal ions, particularly calcium and magnesium, which helps in stabilizing enzymes and preventing metal ion interference in various biochemical assays. It is also employed in buffer solutions to maintain pH stability during experiments involving metal-sensitive processes. In industrial applications, it serves as a component in cleaning formulations to remove metal ions from surfaces and in water treatment processes to control metal ion concentrations. Additionally, it is utilized in the synthesis of coordination compounds and in analytical chemistry for complexometric titrations to determine metal ion concentrations accurately.