EGTA solution is widely used in biological and biochemical research to selectively chelate calcium ions (Ca²⁺) while having minimal effect on magnesium ions (Mg²⁺). This selectivity makes it especially valuable in experiments where precise control of intracellular calcium levels is required, such as in studies of cell signaling, muscle contraction, and neurotransmitter release. It is commonly included in buffer solutions to prevent calcium-dependent enzyme activity, such as proteases and nucleases, thereby protecting proteins and nucleic acids during cell lysis and extraction procedures. EGTA is also used in cell culture applications, particularly in protocols involving cell detachment, where it works synergistically with trypsin by weakening cell adhesion through calcium removal. In electrophysiology, EGTA is added to intracellular pipette solutions to buffer calcium and study ion channel behavior under controlled conditions. Additionally, it is employed in assays involving calcium-sensitive dyes or proteins to establish baseline measurements without calcium interference. Its ability to stabilize experimental conditions makes EGTA an essential tool in molecular and cellular biology.