Used primarily in biological and biochemical research to chelate calcium ions with high selectivity over magnesium ions. This property makes it valuable in experiments involving cell signaling, where precise control of intracellular calcium levels is required. Commonly added to buffer solutions to prevent calcium-dependent enzyme activity, such as proteases and nucleases, during cell lysis or protein extraction. Also employed in studies of muscle contraction, neurotransmitter release, and apoptosis to modulate calcium-triggered processes. Frequently used in electrophysiology and cell culture protocols to isolate the effects of calcium from other divalent cations.