N-Acetylthiourea is primarily used in organic synthesis as a versatile intermediate. It plays a significant role in the preparation of various heterocyclic compounds, which are essential in pharmaceutical and agrochemical industries. Additionally, it is employed in the synthesis of thiazole derivatives, which have applications in medicinal chemistry due to their biological activities. The compound is also utilized in research settings for studying reaction mechanisms and developing new synthetic methodologies. Its ability to act as a sulfur donor makes it valuable in the production of sulfur-containing organic molecules.