This chiral thiourea derivative is a highly effective organocatalyst for asymmetric synthesis. The 3,5-bis(trifluoromethyl)phenyl group provides strong Brønsted acidity and hydrogen-bond donation, while the (1S,2S)-2-(1,3-dihydro-2H-isoindol-2-yl)cyclohexyl moiety ensures excellent chirality induction. It excels in enantioselective reactions such as Michael additions, aza-Henry reactions, Mannich reactions, and Pictet-Spengler cyclizations, delivering high yields and enantioselectivities often exceeding 99% ee. With ≥98% purity and ≥99% ee, it is indispensable for preparing enantiopure intermediates in medicinal chemistry, natural product synthesis, and fine chemicals production.