Used primarily as a fluorescent probe in biochemical research, it aids in the study of protein interactions and conformational changes due to its strong fluorescence properties. It is also employed in the detection of metal ions, particularly copper and mercury, in environmental samples, leveraging its sensitivity to these elements. Additionally, it serves as a derivatizing agent in chromatography to enhance the detection of thiol-containing compounds, improving analytical accuracy in complex mixtures. Its application extends to the development of sensors for biological and environmental monitoring, where precise detection is crucial.