2-Methyl-3-thiosemicarbazide is primarily used in the synthesis of various heterocyclic compounds, which are valuable in medicinal chemistry and drug development. It serves as a key intermediate in the preparation of thiadiazole derivatives, known for their antimicrobial, antifungal, and antiviral properties. Additionally, this compound is utilized in coordination chemistry to form metal complexes, which are studied for their potential applications in catalysis and material science. Its role in organic synthesis also extends to the development of agrochemicals, contributing to the creation of pesticides and herbicides. Furthermore, 2-methyl-3-thiosemicarbazide is employed in analytical chemistry as a reagent for the detection and quantification of specific metal ions.