Used as a fluorescent probe for detecting and quantifying antioxidants in cells, particularly glutathione, which plays a crucial role in protecting cells from free radical damage. This compound fluoresces upon reaction with thiol group (-SH) containing substances, making it suitable for molecular biology and biochemistry research, especially in biological systems that require real-time monitoring of intracellular thiol level changes. It works effectively with fluorescence microscopes or fluorescence plate readers.