Used as a fluorescent probe in biochemical and analytical applications, particularly for detecting and quantifying biothiols such as cysteine, homocysteine, and glutathione in biological samples. Its design allows selective reaction with these thiols, resulting in a strong fluorescence signal turn-on, which enables sensitive detection in live cells and physiological environments. Commonly applied in fluorescence imaging, high-throughput screening, and diagnostic assays where real-time monitoring of redox status is required. Also utilized in studying oxidative stress and cellular metabolism due to its responsiveness to changes in thiol concentrations.