N-Benzylmaleimide is widely used in the field of organic synthesis and material science. It serves as a key intermediate in the production of various polymers and resins, particularly in the development of thermosetting plastics. Its reactive double bond allows it to participate in Diels-Alder reactions, making it valuable for creating cross-linked networks in polymer matrices.

In biochemistry, it is utilized as a reagent for modifying proteins and peptides, often acting as a Michael acceptor to selectively target thiol groups in cysteine residues. This property makes it useful in studying protein structure and function, as well as in the development of bioconjugates for drug delivery systems.

Additionally, N-Benzylmaleimide finds applications in the synthesis of pharmaceuticals, where it is employed to construct complex molecules with potential therapeutic effects. Its versatility in chemical reactions makes it a valuable tool in both industrial and academic research.