This is a biotinylated iodoacetamide derivative used in biochemical research as a thiol-reactive crosslinking and labeling agent, particularly for modifying cysteine residues in proteins. The iodoacetamide group enables specific alkylation of thiol groups (-SH), while the biotin moiety allows for high-affinity binding to streptavidin, facilitating purification, detection, and pull-down assays. It is suitable for stabilizing protein structures, conjugating molecules in peptide and protein studies, and preventing disulfide bond scrambling in mass spectrometry workflows. Commonly applied in the development of fluorescent probes, enzyme inhibitors, targeted covalent modifiers in medicinal chemistry, and for labeling thiol-containing biomolecules in bioimaging and targeted drug delivery systems.